Tottenham have applied to the High Court for a judicial review of Newham Council's decision to award the Olympic Stadium to West Ham.
Spurs are unhappy with the process involved with the Hammers successfully securing the tenancy to utilise the venue after the 2012 Olympic Games.
Newham Council had arranged a £40 million loan to finance West Ham's move to the stadium, however, Spurs are unhappy public funds are being used.
The north Londoners now want the High Court to review the process, and only once their lawyers are satisfied will the process will be able to continue.
Spurs have contacted Newham Council directly in order for a response to their issues, however, their questions have been ignored - forcing Wednesday's action.
"The club has on Wednesday sought permission from the High Court to bring a claim against the London Borough of Newham ("Newham") for judicial review of Newham's process in providing a loan for the conversion of the Olympic Stadium after the 2012 Games," read a statement on the club's official website.
"The club wrote to Newham asking it to explain its reasons and justification for its decision, but Newham has declined to respond to this request for information.
"Due to the time limits which apply to claims for judicial review, the club has had no alternative but to issue these proceedings in order to protect its position.
"The club continues to hold discussions with both local and national government bodies in order to seek to agree a feasible stadium solution."