Anton Ferdinand followed his brother Rio and refused to wear a Kick It Out campaign T-shirt ahead of QPR's clash with Everton.
The Rangers defender, dropped to the bench for the game, was joined in his protest by team-mates Shaun Wright-Phillips and Junior Hoilett.
And neither Everton striker Victor Anichebe nor centre-half Sylvain Distin warmed up in the anti-racism organisation's shirt.
Several other players snubbed Kick It Out's initiative over the weekend as the fallout from the John Terry-Anton Ferdinand case continues.
And Anichebe said before the game: "Are we really kicking it out? There's a situation that's just happened. I believe in kicking racism out of everything, not just football - kick it out of society.
"Situations have happened and I don't feel the outcome has been just. I won't wear the T-shirt, I don't think it's right to wear it. If other people want to wear it that's their right.
"I don't want to be some poster boy for Kick Racism Out of Football. You don't really see the T-shirts until something happens, then we decide to wear the T-shirts."
Rio Ferdinand was the lone Manchester United player who did not don the T-shirt ahead of their victory over Stoke on Saturday.
But his stance angered United boss Sir Alex Ferguson, who said afterwards that Ferdinand's decision was "embarrassing" and promised to "deal with it".
PFA chairman and Kick It Out ambassador Clarke Carlisle hopes the decision of some players not to wear anti-racism t-shirts will prompt discussions rather than punishments.
"Sir Alex Ferguson is trying to reaffirm his unwavering support of the Kick It Out campaign and that's fantastic," Carlisle said. "But this should not be seen as player versus club or dissension from a player against their employer.
"This is about a group of players and some wider issues that transcend that relationship.
"We would not want to see Rio Ferdinand punished. As I said of the handshake saga, you cannot coerce any man against his will and to do so would be the complete opposite of what the campaign is for.
"(Reading manager) Brian McDermott and (Newcastle manager) Alan Pardew said they had good conversations with their players to understand why (they did not wear the t-shirt) and they respect them in that.
"Sir Alex Ferguson pointed out in his own interview he did not know why Jason Roberts or any other player would not want to wear the t-shirt, so I hope that conversation takes place in the next couple of days."