Roma defender Matteo Ferrari has suffered an injury to his left knee and will require surgery.
The centre-half is likely to be sidelined for up to three weeks and will definitely miss the second leg of the Italian club's UEFA Champions League quarter-final with Manchester United on Wednesday.
Ferrari had been in contention for a starting role at Old Trafford, but Roma will now be hoping that Brazilian international Juan can shake off a foot problem in time to face the Red Devils.
Christian Panucci deputised in the centre of the defence for the 2-0 first leg defeat at the Stadio Olimpico last Tuesday, but coach Luciano Spalletti is keen to return the former Chelsea man to right-back.
Inspirational club captain Francesco Totti is another doubt for the European encounter after sitting out Roma's last two matches with a muscle tear in his left thigh.
One player who is expected to make the journey to England is Simeone Perrotta, after the midfielder was given the all-clear following a knee injury picked up in training last Thursday.