Cristiano Ronaldo and Nani will miss Portugal's friendly with Greece on Wednesday due to injury.
The Manchester United duo picked up knocks during the Premier League leaders' 3-0 win over Liverpool on Sunday.
Ronaldo, who took his tally to 34 for the season by heading United's second goal against Liverpool, is suffering from pains in his left leg.
Fellow winger Nani came off the bench to fire home his club's third goal at Old Trafford, but misses out for his country after pulling a muscle in his right leg.
Portugal have confirmed the pair will not feature in the midweek friendly and have opted against calling up any replacements.
However, Chelsea defender Ricardo Carvalho, who took a knock to the head in the 2-1 win over Arsenal on Sunday, has been passed fit to play against Greece.