Chesterfield manager Paul Cook has placed Terrell Forbes, Scott Boden and Jack Waddle on the transfer list.
The trio join Neal Trotman and Craig Westcarr in being made available for transfer by Cook.
The Spireites boss told the club's official website: "I see the future of the three lads away from our club so they have gone on the list with a view to a loan or permanent deal.
"To keep the balance within the squad, there must be outgoings to allow players to come in."
Experienced defender Forbes, who started his career at West Ham and later played for QPR, joined Chesterfield in the summer after being released by Leyton Orient.
Striker Boden, 23, signed for the Spireites in 2008 after being released by neighbours Sheffield United.
Waddle, a 19-year-old winger, was handed his first professional contract by the club in the summer.