Chris Coleman has confirmed that Ryan Shawcross has rejected the chance to play for Wales because he wants to pursue an international career with England.
Shawcross, who was born in Chester, had been eligible for a Wales call-up because he spent five years of compulsory education in the country.
Aaron Ramsey had stated that he would have no problem with Shawcross being selected despite having his leg broken by a tackle from the Stoke defender in a Premier League match in 2010.
Wales manager Coleman held talks with Shawcross about the matter earlier this week but was left in no doubt that the 24-year-old remains committed to England.
"I went up to Stoke City this week and I spoke to Ryan," said Coleman.
"I had a good conversation with him and he was very honest - his dream is to play for England.
"He is a good player and we want as many good players available to us as we can so it was disappointing, but I understood his reasons and I really hope he gets his dream of winning an England cap."
Shawcross is not the only person to recently turn down the offer of playing for Wales in the country's World Cup qualifying campaign.
Coleman approached Cardiff defender Ben Turner, who qualifies to play for Wales through his grandmother, but he also wants to pursue his dream of playing for England.
"Ben also sees himself as English," said Coleman.
"I have known him a long time ever since he was a young centre back at Coventry, but there is no further conversation to have there.
"Both avenues have been closed off so we have moved on."