West Ham duo James Collins and Jack Collison have pulled out of the Wales squad to face Bosnia-Herzegovina.
Collins, who rejoined West Ham in the summer from Aston Villa, has withdrawn from Wednesday's friendly with a groin injury.
West Ham midfielder Collison has been forced out with a knee problem.
Wales boss Chris Coleman has called up Huddersfield defender Joel Lynch and Cardiff striker Robert Earnshaw as replacements in his squad.
Team GB quartet Craig Bellamy, Aaron Ramsey, Joe Allen and Neil Taylor are all included in the squad.
Wednesday's game is Coleman's final chance to prepare his squad for the forthcoming World Cup qualifiers.