Kevin Foley has been forced out of the Republic of Ireland's 23-man Euro 2012 squad through injury.
The Wolves defender's place will be taken by Paul McShane, who is in Italy for Ireland's pre-tournament training camp after being drafted in as defensive cover amid a series of fitness concerns.
Foley reported with a hamstring problem and has been working ever since in an effort to shake it off, but he has been forced to withdraw from the squad after failing to prove his fitness.
Foley is the second player to withdraw from Giovanni Trapattoni's original squad after Birmingham's Keith Fahey.
Shay Given and John O'Shea have been included in the squad despite doubts over their fitness.
Trapattoni admitted it was a difficult decision to take to leave out Foley, saying: "Although Kevin has been recovering well, I have made the difficult decision to leave him out of the 23-player list after completing fitness tests on John O'Shea and Paul McShane this morning.
"I have spoken to him and understand how difficult it is, saying that he can remain with the squad if he wishes.
"With the injuries that we experienced in the squad over the past week, I have had to make a tough decision when it comes to naming the 23 players that must be submitted to UEFA for the championship.
"Kevin Foley, John O'Shea, Paul McShane, Darren O'Dea, Shay Given had all been carrying knocks and we have been monitoring them closely in their recovery."
Republic of Ireland squad:
Shay Given (Aston Villa), Keiren Westwood (Sunderland), David Forde (Millwall) John O'Shea (Sunderland), Stephen Kelly (Fulham), Stephen Ward (Wolverhampton Wanderers), Richard Dunne (Aston Villa), Sean St Ledger (Leicester City), Darren O'Dea (Leeds United), Paul McShane (Hull City); Glenn Whelan (Stoke City), Keith Andrews (West Bromwich Albion), Paul Green (unattached), Darron Gibson (Everton), Damien Duff (Fulham), Aiden McGeady (Spartak Moscow), Stephen Hunt (Wolverhampton Wanderers), James McClean (Sunderland), Robbie Keane (LA Galaxy), Kevin Doyle (Wolverhampton Wanderers), Shane Long, Simon Cox (both West Bromwich Albion), Jon Walters (Stoke City)