Mick McCarthy and his Wolves players have slammed Wigan defender Antolin Alcaraz for appearing to spit at Richard Stearman during Sunday's clash between the sides at Molineux.
The incident occurred in the 83rd minute of Wolves' crucial 3-1 home victory and sparked a brawl involving several players from each team.
Referee Lee Probert took no action but Stearman confirmed that Alcaraz had spat in his direction after it was initially thought it was aimed at Christophe Berra.
Stearman was reluctant to talk about the incident but said: "It wasn't Berra, I'll give you that, but I don't think it's right for me to talk about it at the moment."
Asked if he had been spat at, Stearman added: "Yes, but I'm not going to speak about it."
McCarthy said: "Spitting is taboo if that's what happened. I only had it happen to me once and the player didn't come too close to me for a long time after that.
"If it has been shown, that is unacceptable behaviour and anyone here who was spat at would act accordingly.
"If that has happened, he shouldn't be doing it."
David Edwards, who scored Wolves' second goal, said: "That is out of order. That shouldn't be brought into the game and hopefully he should be pulled up on that.
"I'm sure with the television footage as well, some sort of action should be taken."
Wigan boss Roberto Martinez would only say: "I haven't seen (the replays) yet. If that is the case, I'll look into it and I'll find out."