Northern Ireland goalkeeper Lee Camp and midfielder Grant McCann will miss Tuesday's final Euro 2012 qualifier against Italy.
The duo sustained groin injuries in Friday's defeat to Estonia at Windsor Park and Nigel Worthington admitted after the game they were doubtful for the trip to Pescara.
It is now been confirmed that Camp and McCann will not be fit, leaving Northern Ireland to face the prospect of visiting the Group C winners with a heavily weakened team.
Already without skipper Aaron Hughes (groin) and fellow defender Jonny Evans (ankle) for the Estonia defeat, under-pressure manager Worthington must now fill the void left by Camp, McCann and striker Kyle Lafferty.
The latter is suspended after picking up a second yellow card of the campaign.
A short statement released by the Irish Football Association read: "Lee Camp and Grant McCann have been ruled out of Northern Ireland's final Euro 2012 qualifier against Italy on Tuesday.
"The pair will miss the game as a result of groin injuries."
Coventry's Sammy Clingan is another doubt after coming off in the 32nd minute with a back problem against Estonia, but he will travel.
Camp's injury opens the door for veteran goalkeeper Maik Taylor to make what is sure to be a farewell appearance for the national side.
The 40-year-old is currently unattached after his release by Birmingham and lost his Northern Ireland spot earlier this year when former England Under-21 Camp switched his allegiance.
He is unlikely to announce his retirement but it would be a surprise if Worthington - or any successor - kept him around for the World Cup 2014 qualifying campaign.