Sky Sports News understands that Alan Hutton is facing a month on the sidelines due to a knee problem.
Tottenham manager Harry Redknapp had already been dealing with a defensive injury crisis and news of Hutton's lay-off will come as a major blow.
The Scottish right-back, who has played 27 times for the club this season, looks set to miss a large chunk of Spurs' run-in.
He will sit out both legs of the UEFA Champions League quarter-final against Real Madrid - the first of which is in the Spanish capital on Tuesday - as well as several Premier League fixtures.
Ledley King, Jonathan Woodgate and Younes Kaboul are all injured at present, while William Gallas is a serious doubt for Saturday's clash with Wigan and Tuesday's game in the Bernabeu because of a knee problem.
Michael Dawson and Sebastien Bassong are Redknapp's only two fit natural centre-halves, while Hutton's rival at right-back, Vedran Corluka, can also play there if needed.