Wolfsburg midfielder Diego has been suspended by the club for one match after defying orders.
The 25-year-old Brazil international took a penalty during their 1-0 defeat to Hannover on Saturday, despite the fact that he is not the designated spot-kick taker.
Diego crashed his effort against the crossbar and Wolfsburg went on to lose 1-0 in Steve McClaren's last game in charge.
New coach Pierre Littbarski is expected to take the reins until the end of the season and confirmed that Diego would play no part against Hamburg this weekend.
"Diego will not play. I have suspended (him) following the incident in our most recent game," he said.
"After Saturday it will all be forgotten and he can show us again if he can be of help to us."
Wolfsburg opted to part company with McClaren after the former England boss could only lead the Bundesliga outfit to 23 points from 21 games.