Darren Fletcher insists Manchester United's focus has always been on winning the Premier League title following the end of the club's unbeaten run.
United's record-equalling spell of 29 league games without defeat was brought to a halt on Saturday following a shock 2-1 reverse at struggling Wolves.
The setback at Molineux put a dent in the Red Devils' title bid, but Fletcher is keen to see his team-mates bounce back in next weekend's Manchester derby.
"The main concern for us is winning the league and losing today is a dent in our challenge," the midfielder told MUTV.
"That is the real disappointment for the lads. The unbeaten record has gone and people will stop talking about that now.
"We could have remained unbeaten the whole season and not won the league. What would have been the point in that?
"Our focus is to win the league. We have lost three points and we will have to bounce back and show our character."
United had taken an early lead at Wolves following Nani's goal and Fletcher was disappointed at the failure to respond after the home side had gone ahead through a combination of George Elokobi and Kevin Doyle.
He added: "It was a great start for us and from moments like that we should really go on and see the game out. We had a couple of chances in the first half, didn't take them and conceding goals from set-pieces ultimately cost us.
"We are obviously disappointed to lose after getting off to a great start. We knew it would be a difficult game against Wolves, they play a high tempo physical game and the ball is always in the air.
"We just didn't create that chance in the second half to get us back in the game."