John Terry, Ashley Cole and Gabriel Agbonlahor have been ruled out of England's friendly against France on Wednesday because of injury.
Bolton's Gary Cahill and Aston Villa full-back Stephen Warnock have been drafted as replacements for Terry and Cole.
Chelsea captain Terry missed his side's shock home defeat to Sunderland on Sunday with a hamstring problem.
Blues boss Carlo Ancelotti confirmed after the game Terry was set to miss the friendly and he has now been forced to pull out of the showdown with France at Wembley.
Cole played the full 90 minutes of the defeat to Sunderland, but he has withdrawn from the squad with an unspecified problem.
The left-back's absence could mean Arsenal defender Kieran Gibbs is handed his first senior start for his country.
Aston Villa striker Agbonlahor was rated doubtful after picking up a knock against Manchester United on Saturday and he has been now forced to pull out of the squad.
West Ham forward Carlton Cole has been drafted in to fill the void left by Agbonlahor's withdrawal.
Newcastle striker Andy Carroll remains a doubt with a minor groin injury and he has joined up with the squad to have his injury assessed by the England medical staff.