The Football Association has confirmed that Phil Jagielka has been ruled out of England's Euro 2012 qualifier against Montenegro with a hamstring injury.
Bolton's Gary Cahill has been called into the squad as his replacement ahead of Tuesday's match at Wembley.
Jagielka trained with England on Friday morning, but a scan has since highlighted his injury and he has returned to Everton for treatment.
The fitness setback will be a cause for frustration for the former Sheffield United defender after his impressive performances in England's last Euro 2012 qualifiers.
Jagielka stepped into the injured boots of Rio Ferdinand to produce solid displays against Bulgaria and Switzerland.
He was therefore expected to keep his place against Montenegro, but he will now be unable to make immediate progress.
Additionally, the FA has announced that Manchester City midfielder Gareth Barry will be reporting to the team hotel on Friday evening after being given additional time at home for personal reasons.