Mexico internationals Carlos Vela and Efrain Juarez have been handed six-month bans by their national federation.
The pair allegedly threw a party at a team hotel following a friendly against Colombia earlier this month.
As part of the crackdown on the breach of discipline, 11 other players were also fined for their part in the incident.
The sanctions were announced by national team director Nestor de la Torre, who confirmed that Arsenal forward Vela and Celtic midfielder Juarez violated four rules from the federation's code of conduct after the 1-0 win in Monterrey.
De la Torre said: "Carlos Vela and Efrain Juarez will be suspended for six months from the date of these events [8th September], for calls for the national team, because there were faults in four sections of the national team rules."
The 11 players to receive fines of 50,000 pesos (£2,517) were Giovani dos Santos, Javier Hernandez, Guillermo Ochoa, Francisco Rodriguez, Carlos Salcido, Andres Guardado, Gerardo Torrado, Pablo Barrera, Rafael Marquez, Enrique Esqueda and Hector Moreno.