Mexico coach Javier Aguirre has named his final 23-man party for the World Cup this summer.
Aguirre selected the final line-up from his provisional squad of 26, with Barcelona midfielder Jonathan dos Santos among those who did not make the cut.
The 20-year-old, who played the full 90 minutes of Mexico's 5-1 friendly thrashing of Gambia on Sunday, suffered a muscle tear while training with Barca last month which limited his playing time ahead of Aguirre's selection.
A statement from the Mexico football federation on Monday said: "The Barcelona player Jonathan dos Santos has been removed from the squad and will not participate at the World Cup."
Also axed from the line-up are defenders Juan Carlos Valenzuela and Adrian Aldrete.
Meanwhile, Dos Santos' older brother Giovani has made the final squad, while forwards Guillermo Franco of West Ham and Arsenal's Carlos Vela are also included.
Memo Ochoa (America), Luis Ernesto Michel (Chivas), Oscar Perez (Chiapas); Rafael Marquez (Barcelona), Ricardo Osorio (Stuttgart), Hector Moreno (AZ Alkmaar), Francisco Rodriguez (PSV Eindhoven), Carlos Salcido (PSV Eindhoven), Paul Aguilar (Pachuca), Efrain Juarez (Pumas); Jonny Magallon (Guadalajara), Jorge Torres Nilo (Atlas), Gerardo Torrado (Cruz Azul), Israel Castro (Pumas), Andres Guardado (Deportivo La Coruna); Pablo Barrera (Pumas), Adolfo Bautista (Guadalajara), Alberto Medina (Guadalajara), Cuauhtemoc Blanco (Veracruz), Javier Hernandez (Guadalajara), Giovani dos Santos (Galatasaray), Guillermo Franco (West Ham), Carlos Vela (Arsenal).