Hampshire suffered their first defeat in 17 Friends Life t20 matches when they were beaten by eight runs by Kent at the Ageas Bowl.
The defending champions could only muster 135-8 following a late collapse as they chased the Spitfires' total of 143-8 in the South Group match.
The Royals' previous defeat in the competition also came at the hands of Kent in Southampton, when they went down by three runs in June 2012.
Kent, who had lost their five previous Twenty20 matches this season, handed competition debuts to Daniel Bell-Drummond and Fabian Cowdrey.
The changes paid dividends with the pair sharing a bright 55-run opening partnership in seven overs before Danny Briggs bowled Cowdrey (21) and, in his next over, had Bell-Drummond (27) caught at deep mid-wicket.
Briggs (3-19) then dismissed Darren Stevens and Kent lost wickets in clusters after that, with Alex Blake and Geraint Jones falling in quick succession to leave the visitors 94-5 in the 14th over.
Sam Billings (26) and Vernon Philander (14) improved Kent's position, but Sohail Tanvir (3-29) returned for his second spell to cut through the lower order and leave the Spitfires defending a below-par total.
Hampshire were behind the required run rate throughout their innings and lost James Vince (5) and Jimmy Adams (13) to slip to 50-2.
Michael Carberry (35) hit the first six of the match and four boundaries before he was third man out with the score on 64.
The asking rate had crept up to 11 runs per over by the start of the 16th over when Sean Ervine (27) launched an 18-run assault on Stevens.
Ervine and Neil McKenzie (47) took another 16 runs off the 17th over by Philander to lift Hampshire to 124-3 and to within 20 runs of victory.
But with Mitchell Claydon (2-24) remaining calm, Hampshire completely lost their way - the last five wickets falling for nine runs as they came up eight runs short.