Brett Lee has lost his battle to be fit for the third Ashes Test against England, which starts at Edgbaston on Thursday.
The Australian paceman was already regarded as doubtful with the side strain that had kept him out of the opening two games in Cardiff and at Lord's.
Vice-captain Michael Clarke said: "I don't think that Brett is going to be right for Thursday. He only bowled off three or four steps today in the net.
"He is doing plenty of fitness work and is working very hard to get himself right.
"I am not sure exactly how far away he is but I think he is out of contention for Thursday."
Meanwhile, Clarke said he was pleasantly surprised after taking his first view of the Edgbaston wicket on Tuesday.
He added: "The wicket looks better than we expected. It is quite dry. I would imagine that spin will play a part in this game.
"I don't think the forecast is great for the rest of the week and I am sure conditions will come into the equation when selecting the side.
"We know we need to improve with bat and ball and perform at the highest level."