England will wait until after Thursday's final practice session before making a decision on Stuart Broad's fitness for the first One Day International in Rajkot.
The Nottinghamshire all-rounder was injured in Tuesday's humiliating defeat by Mumbia and with Ryan Sidebottom definitely out of the opener with a longstanding Achilles problem, England face an anxious wait over Broad.
Coach Peter Moores told Sky Sports News: "Stuart Broad picked up a knee injury in the first practice game, he landed awkwardly trying to stop a boundary.
"It has swollen up quite a lot, he got it injected and it seems to have settled down. We won't know properly if he is fit for that first ODI until he bowls on Thursday in practice. Providing he comes through that, he will be available."
As for that defeat by Mumbai, Moores insists it has merely re-emphasised what a tough tour England are facing.
"The lessons you learn are that in sports you must respect everything about the game, the opposition, how it is going on, or whatever," he said.
"That is the most important thing. The real lesson for us is that it is going to be tough. We know it is going to be tough starting on Friday.
"We have got another two days to get ourselves ready. But we are playing some good cricket, we have got some good batters. We lost our top order (against Mumbai). The key will be by Friday we are all feeling good, we are confident, we are ready to go."
England's inept batting display against Mumbai has left a number of key players distinctly short of batting practice heading into Friday's series opener.
Captain Pietersen has only faced 23 balls in his two Indian innings, Paul Collingwood only 37 and opener Matt Prior just 24 balls.
All-rounders Luke Wright and Ravi Bopara, who are fighting for one place in the middle order, have also failed to occupy the crease for any substantial period, but Moores insists there is no cause for concern.
"It's not a massive concern because they think they're playing well and it's happened before and it's not affected how they've played," he explained.
"The captain, for instance, has always been a big-match player, he's hitting it very well and I don't expect it to make much difference to how he plays and how he feels about going to play.
"In an ideal world, everyone would have had time in the middle, everyone is feeling great but it doesn't always happen like that.
"The key issue is for me, as a team, we are confident in how we're going to play our cricket and I don't think that is affected by Tuesday at all."