Matthew Hayden has become the latest player to pull out of Australia's squad for the one-day series against Bangladesh.
The left-handed opener is still recovering from an Achilles injury he picked up while playing in the Indian Premier League.
He had originally been named in the 14-man party for the three matches in Darwin, despite not being fully fit.
However, his withdrawal means the hosts will now be without three of their big guns. Skipper Ricky Ponting and pace bowler Brett Lee had already been ruled out.
Cricket Australia have announced that they will not call up a replacement for Hayden, who is still expected to be fit in time for the Test series against India in October.
Despite the big name absentees stand-in skipper Michael Clarke insists they will not be taking Bangladesh lightly.
"They beat us in Cardiff (in the NatWest Series in 2005), and that sits in my memory," he said.
"We will certainly speak about that when we arrive in Darwin. We're going to have to play good cricket."
The opening match takes place on Saturday with the second on Wednesday September 3 and the final fixture on September 6. All three games are to be held at the Marrara Cricket Ground.
The one-dayers against the Tigers had been scheduled to aid Australia's preparations for the ICC Champions Trophy, which has now been postponed until 2009.
Clarke was relieved that a decision had finally been made about the tournament following several teams citing concerns over security in Pakistan.
"I think my view has been all the time that the ICC, Cricket Australia and the ACA (Australian Cricketers Association) would make a decision and they have finally done that," the batsman said.
"It's a tournament Australia currently holds. So, come next year whenever they put it back on, we are looking forward to retaining that trophy.
"Right now I think all the guys are rapt that the ICC has made a decision."