Pakistan captain Shoaib Malik twisted his ankle while playing football following their loss in the opening Test against India in Delhi.
"The boys were playing football after the match when Malik twisted his ankle. He was taken to a hospital for precautionary X-rays," Pakistan team media co-ordinator Javed Akhtar said.
Akhtar says it is too early to say whether Malik would be doubtful for the second Test, starting on Friday.
Pakistan are already without fast bowlers Mohammad Asif and Umar Gul for the second game in Kolkata.