Stuart Broad, Tim Bresnan and Graeme Swann will all sit out England's third and final one-dayer against the West Indies at Headingley.
After taking an unassailable 2-0 lead with a comprehensive eight-wicket victory at the Oval, England's selectors have opted to take the chance to look at some fresh faces on Friday.
Seam duo Bresnan and Broad and off-spinner Swann have all been given the chance to rest ahead of the upcoming series against Australia.
Surrey paceman Stuart Meaker and Kent spinner James Tredwell have been called up, while Warwickshire's Chris Woakes will continue to be part of the squad after being drafted in on the eve of the second ODI.
Woakes was summoned to cover for Jade Dernbach, who was offered the option of compassionate leave for the remainder of the series following the death of his friend and Surrey team-mate Tom Maynard on Monday.
National selector Geoff Miller said: "The fact that we have already won the NatWest series means we are able to take the opportunity to rest three players ahead of next week's series against Australia and take a closer look at players who are likely to feature in our limited overs planning going forward."
England took the decision to leave James Anderson out of their squad for the final Test against the same opponents earlier this summer, a match Broad also eventually sat out after the opening two days had been lost to bad weather.
Twenty20 captain Broad, as well as Bresnan and Swann, are expected to return for England in the one-off T20 clash against the West Indies on Sunday.