Nicola Adams is set to undergo surgery and will miss the Women's World Boxing Championships in South Korea in November.
Adams had been desperate to claim the one major title that still eludes her after three consecutive world silver medals in 2008, 2010 and 2012.
But the Olympic and Commonwealth champion is now due to have an operation on a shoulder injury in the next two weeks which will keep out of the ring for up to six months.
She said: "I struggled through the Commonwealth Games so winning gold for England meant even more to me knowing I was not competing at my best.
"Choosing to have the operation now was not an easy decision. I am gutted not to be taking part in the World Championships, as I know everyone was behind me.
“But having the operation now will mean I have longer to recover and will be able to return fitter and stronger."
Adams was pushed to the limit by Northern Ireland's Michaela Walsh in the women's flyweight final in Glasgow earlier this month, eventually winning via a split decision.
GB Boxing Performance Director Rob McCracken said: "It became apparent during the Commonwealth Games that Nicola's shoulder was deteriorating as the competition went on.
"Nicola did extremely well to still win the gold medal. However we were not confident her shoulder would stand-up to another two week competition so decided it was better to have the operation now.
"The Rio Olympics is our top priority and we want to ensure Nicola is recovered and back to full fitness come 2016."