Roma midfielder Daniele De Rossi has been omitted from the Italy squad for Wednesday's friendly against Spain in Madrid for breaking head coach Cesare Prandelli's code of ethics.
Azzurri vice-captain De Rossi was left out after punching Inter Milan striker Mauro Icardi during Saturday's goalless Serie A match in Rome.
Although not spotted by Italian referee Mauro Bergonzi, the incident was captured on television footage.
De Rossi has been handed a retrospective three-match ban for the punch.
A Lega statement confirmed the "punch to the face" had not been detected by match officials and after taking De Rossi's "deliberate" demeanour into account, deemed the foul to be violent conduct.
The 30-year-old will now miss games against Napoli, Udinese and Chievo while his club Roma bid to catch leaders Juventus - who hold an 11-point advantage - over the season run-in.
Inter defender Juan Jesus was also handed a retrospective three-match ban after the Lega reviewed footage of him punching Roma counterpart Alessio Romagnoli. Referee Bergonzi did not see the incident.
Sunderland midfielder Emanuele Giaccherini was among the 26 players included in Prandelli's squad announced by the Italian federation on Sunday evening. The 28-year-old has scored three goals in 15 Barclays Premier League appearances for the Black Cats this term.
Torino striker Ciro Immobile and the Parma duo of defender Gabriele Paletta and midfielder Marco Parolo have also been rewarded for their strong performances this season with international call-ups.
However, Mario Balotelli misses out due to a shoulder injury.
Italy squad for friendly against Spain in Madrid on March 5: Buffon (Juventus), Perin (Genoa), Sirigu (Paris St Germain); Abate, Astori (Cagliari), Barzagli (Juventus), Bonucci (Juventus), Chiellini (Juventus), Criscito (Zenit St Petersburg), De Sciglio, Maggio (Napoli), Paletta (Parma); Candreva (Lazio), Giaccherini (Sunderland), Marchisio (Juventus), Montolivo (AC Milan), Thiago Motta (Paris St Germain), Parolo (Parma), Pirlo (Juventus), Verratti (Paris St Germain), Cerci (Torino), Destro (Roma), Gilardino (Genoa), Immobile (Torino), Insigne (Napoli), Osvaldo (Juventus).